Targeted and complete analyses
Choose your method:
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Metabarcoding:
16S: V1-V3; V5-V6; V4; V3-V4
ITS: ITS1-ITS2; ITS1-ITS4
18S: choice of 563F, 574F, 1132R, 1391F, EuKB_rev
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Shotgun metagenomics (WGS)
Metatranscriptomics (RNA-Seq)
Complete Service
Send us your gDNA
samples and make use of
our Complete Service:
Purification of gDNA / RNA from various starting material. Please
enquire.
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Optimized protocols for depletion of unwanted
sequences:
rRNA depletion
host mRNA depletion
mitochondrial / plastid 16S rRNA
Optimized protocols for depletion of unwanted sequences:
rRNA depletion
host mRNA depletion
mitochondrial / plastid 16S rRNA
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MiSeq 2x300 min 50.000 fragments:
12-23 samples = 58 €/sample
24-47 samples = 52 €/sample
48-96 samples = 48 €/sample
96+ samples = 46 €/sample
PNA blockers = +4 €/sample
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NovaSeq 2x250bp, 100.000+ fragments:
384+ samples = 26 €/sample
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WGS 2x150bp, min 5M reads:
12-23 samples = 132 €/sample
24-48 samples = 95 €/sample
48-96 samples = 74 €/sample
+ 96 samples = 62 €/sample
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Metatranscriptomics, 30M reads:
Starting from 381€/sample
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* For variable depth or different multiplexing please enquire.
Your libraries
Sequence your custom libraries:
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Miseq run (300PE)
3.330 €
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Novaseq 1000M reads (150PE)
2700 €
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Novaseq 650M reads (250PE)
3900 €
Includes:
- Free Illumina App analysis for Mi Seq samples (on request)
- Library normalization (up to 192 samples)
Bioinformatics pipeline
From taxonomic abundance to integrated diversity analyses:
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Amplicon Analysis
First 12 samples = 500 €
13-24 samples = 33 €/sample
25-36 samples = 28 €/sample
36+ samples = 25 €/sample
96+ samples = 20 €/sample
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* For WGS Analysis please enquire.
ASV and OTU clustering, taxonomy, assignment, alpha/beta diversity, PCoA and taxonomic enrichment testing (on request). Sample stratification on metadata factors.
Details:
- Metagenomics / Metatranscriptomics analyses are based on HUMAnN2 framework
- Amplicon Analysis (OTU or ASV)
- Metabarcoding pipeline based on QIIME or DADA2
- Custom database analyses can be run with LCA (lowest common ancestor) BLAST
