RNA-Seq

One MOLECULE, INFINITE TOOL

Targeted depletion of unwanted transcripts results in a significant reduction in sequencing reads derived from cytoplasmic and mitochondrial rRNAs, globin, chloroplasts, housekeeping genes, or any other transcript species that may not be relevant to a study, for more efficient use of sequencing resources.

As a part of our standard procedure we prepare strand oriented RNA-seq “libraries” that maintain the information on which strand the original mRNA template is coming from, allowing to accurately determine gene expression from overlapping genes or to discover antisense regulators.

Tools for efficient cDNA generation from ultra-low inputs supporting research in areas previously hampered by quantity are part of our portfolio.

For the high-throughput single cell RNA-Seq we use 10x Genomics Chromium System that permits to perform deep profiling of heterogeneous populations with digital gene expression on a cell-by-cell basis ensuring that biologically relevant signals of individual cells are not masked by bulk average measurements.

Long-reads sequencing platform (Nanopore) is used in the context of projects where full lenght transcripts are sequenced to obtain fine structure of iso-form to study their differential expression ot to fine annotate de novo assembled genomes. This can be achieved either via sequencing of amplified full-length cDNA in a multiplexed fashion or access the RNA sequence directly with the RNA004 kit from Nanopore (including post-trascriptinal epigenetic modifications).

Analysis of RNA-binding protein immunoprecipitation that maps RNA-protein associations in vivo by discovering genome-wide RNA molecules of any type that physically interact with a regulatory protein or protein complex. This as many other custom bioinfomatics analyses can be performed on request. Please enquire

We also provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements, and will also be your technical resource and point of contact for the length of your project. See bioinformatics.