During the years, IGATech has collborated with several research groups and experts to build optimized targeted genotyping panels with the Allegro technology (also known as SPET). Similar to GBS but with the unique ability to target specific SNPs/loci or gene of interest. These panels have been specifically curated either by our team or by researchers with strong expertise in the genetics of the subject species. The panels listed below are readily available and can be used with a minimum order of one plate. Being already in stock, the turnaround times will be much faster as compared to new custom designs.
Note that this is a sequencing-based genotyping and therefore panels are capable to retrieve also non-target polymorphisms. This makes the panels highly reusable also on populations that are far from those that were originally exploited to target specific SNPs. Each locus will be sequenced for hundreds of base pairs (usually about 300bp).
Crop Species
Maize (Zea mais) - 50k loci
The 50k maize panel was generated with the collaboration of IGA and IGATech within the framework of the Novabreed project. It has been highly curated as the results of a two tier approach where the 50k probes have been selected over a pilot panel of about 100k probes. This design has been largely tested in the genotyping of a MAGIC population in collaboration with the Crop Genetics Group of Scuola Superiore Sant'Anna (Pisa). See our webinar in collaboration with Tecan Genomics on the genotyping of MAGIC lines and the seminal paper on SPET technology where we presented the benchmark of the maize panel against the Illumina Infinium chip.
Fava bean (Vicia faba) - 90k loci
Developed in collaboration with Department of Molecular Biology and Genetics, Aarhus University and the Department of Crop Science, Reading University
Fruits and Vegetables
Grape (Vitis vinifera) - 60k loci
The 60k grape panel has been originally delevoped in collaboration with Institute for Adriatic Crops and Karst Reclamation. It mostly cover all genes of the grape genome with homogeneous distribution. Given the high veriability of the genome and to maitain reusability also in wild species, probes have been mainly designed on coding regions while allowing to sequence also more variable regions such as introns.
Tomato (Solanum lycopersicum) - 10k loci
Developed in collaboration with ISI Sementi and CREA - Centro di Ricerca in Orticoltura e FLorovivaismo , Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia
Tomato (Solanum lycopersicum) - 5k loci
Developed in framework of the G2P-SOL project the Tomato 5k has been used to genotype more than 16000 accessions to characterize worldwide genebanks. It is the result of the selection from a pilot design from which the most informative loci were selected.
Eggplant (Solanum melongena) - 5k loci
Developed in framework of the G2P-SOL project the Eggplant 5k has been used to genotype more than 4000 accessions to characterize worldwide genebanks. It is the result of the selection from a pilot design from which the most informative loci were selected.
Peach (Prunus persica) - 30k loci
Developed in collaboration with University of Milano, Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia. Half of the selected loci have overlap with SNPs present in the Peach 18K SNP array (link).
Apricot (Prunus armeniaca) - 25k loci
Developed in collaboration with University of Milano, Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia).
Lettuce (Lactuca sativa) - 40k loci
The 40k lettuce panel was developed in collaboraton with The European Cooperative Programme for Plant Genetic Resources (ECPGR).
Lemon (Citrus lemon) - 30k loci
Developed in collaboration with University of Catania, Dipartimento di Agricoltura, Alimentazione ed Ambiente.
Coffee (Coffea arabica) - 50k loci
The 50k Coffea arabica genotyping platform was built on the purpose of a collaborative effort with Illycaffè S.p.A. with the purpose of analyzing the genetic basis of resistance genes to coffea leaf rust. Probes are uniformily spread across the genome and can provide segregation analysis in both Coffea eugenioides and Coffea canefora sub genomes in the tetraploid Coffea arabica genome.
Forest Trees
Black poplar (Populus nigra) - 5k loci
A selection of 5k higly informative loci utilized in the Epidiverse project and extracted from the first prototype of SPET technoogy (50k panel) within the Watbio project. The panel has been designed taking into account the whole diversity of European populations.
FORGENIUS European silver fir (Abies alba) - 10k loci
FORGENIUS European silver fir (Pinus halepensis) - 10k loci
FORGENIUS European black pine (Pinus nigra) - 10k loci
FORGENIUS European crab apple (Malus sylvestris) - 10k loci
FORGENIUS European yew (Taxus baccata) - 10k loci
FORGENIUS European ash(Fraxinus excelsior) - 10k loci
FORGENIUS European white birch (Betula pendula) - 10k loci
FORGENIUS Wild cherry (Prunus avium) - 10k loci
COMING SOON
- Picea Abies (10k)
- Fagus sylvatica (10k)
- Sorbus torminalis (10k)
- Quercus robur + Q. ilex (10k)
- Castanea sativa (10k)
- Juniperus communis (10k)
- Pinus heldreichii, P. tabuliformis, P. pinaster (10k)
Animals
Goat (Capra hircus) - 4k loci
A 4k (double-probe) panel designed in collaboration with University of Bari, Department of Veterinary Medicine. Most of the sites covers SNPs present in the GoatSNP50 chip, supplemented with coverage on caseine genes.
End of Life designs
End of Life design will be served until the stock of probes will be exhausted. Please enquire to know the amount of residual assays.
Scots pine (Pinus sylvestris) - 8k loci (EOL)
The 8k Pinus sylvestris panel was generated in collaboration with INIA, Forest Research Center (Chile).
European yew (Taxus baccata) - 10k loci (EOL)
The 10k Taxus baccata panel was generated in the framework of the GenTree project.The goal of GenTree is to provide the European forestry sector with better knowledge, methods and tools for optimising the management and sustainable use of forest genetic resources (FGR) in Europe in the context of climate change and continuously evolving demands for forest products and services.
European black pine (Pinus nigra) - 10k loci (EOL)
The 10k Pinus nigra panel was generated in the framework of the GenTree project.The goal of GenTree is to provide the European forestry sector with better knowledge, methods and tools for optimising the management and sustainable use of forest genetic resources (FGR) in Europe in the context of climate change and continuously evolving demands for forest products and services.
European silver fir (Abies alba) - 10k loci (EOL)
The 10k Abies alba panel was generated in the framework of the GenTree project.The goal of GenTree is to provide the European forestry sector with better knowledge, methods and tools for optimising the management and sustainable use of forest genetic resources (FGR) in Europe in the context of climate change and continuously evolving demands for forest products and services.
Jerusalem pine (Pinus halepensis) - 10k loci (EOL)
The 10k Pinus halepensis panel was generated in the framework of the GenTree project.The goal of GenTree is to provide the European forestry sector with better knowledge, methods and tools for optimising the management and sustainable use of forest genetic resources (FGR) in Europe in the context of climate change and continuously evolving demands for forest products and services.